PRODUCT CODE:

121170

Methylene Blue (C.I. 52015) PA

C₁₆H₁₈ClN₃S.xH₂O

M.= 319,85+H2O	CAS [61-73-4]	EINECS 200-515-2
NC 3204 13 00

SYNONYMS: 3,7-Bis(Dimethylamino)Pheno-5-thiazinium Chloride · Tetramethylthionine Chloride · C.I. Basic Blue 9 ·
PHYSICAL DATA: Dark green with bronze luster crystalls or crystalline powder · Solubility in water at 20 °C 40 g/l. In alcohol 15 g/l · Insoluble in ether · Soluble in trichloromethane · M. P.: 180 ºC · Thermal decom. above 180 ºC ·
APPLICATIONS: Analytical reagent: USP 31 · BP 2008 · Ph. Eur. 6.0 · Redox indicator · Preparation of mixtures of stains · Stain for RNA and ribonuclease in electrophoresis; Peacock, A.C. et. al., Biochem, 6 , 1818 (1967); Wolf G.; Epenentia , 24 , 890 (1968) · Starting material for the preparation of azures; Wittekind, D.H. Gehring T. Histochem. J. , 17, 263-289 (1985) · Used in bacteriology , histology , cytology and hematology; Clark G. "Staining Procedures", 4 th Ed. Williams and Wilkins , Baltimore , (1981) ·
BIBLIOGRAPHY: Merck Index 12 , 6137 13 , 6085 · Safety 2 , 2344 C · Römpp 8 , 2586 · Beilstein 27 , 393 IV, 5152 · BRN: 3599847 · Conn’s IX , 423 , 604 · USP 31 · BP 2008 · Ph. Eur. 5.0 (2005) 6.0 (2008) ·

HAZARDOUS: RTECS: SO 5600000 ·

R: 22

SPECIFICATIONS:

Assay (Spectrophotometric)	82 %
Identity	IR p/t.
l of max. ABS in H₂O	663-667 nm
A 1%, 1 cm, lmax. (calc. a.d.s.)	>2200
T.L.C	p/t.
MAXIMUM LIMIT OF IMPURITIES
Loss on drying at 110°C	8-16 %